Biography
Dr. Shadab A. Siddiqi is a Professor of Medicine in the Burnett School of Biomedical Sciences at the University of Central Florida College of Medicine. He received his Ph.D. in Protein Chemistry from the Central Drug Research Institute and Lucknow University, India, followed by postdoctoral training in Cell Biology at the National Institute of Immunology, New Delhi, and in Intracellular Lipid Transport at the University of Tennessee Health Science Center, Memphis.
Dr. Siddiqi joined UCF in 2009 and established an independent research program focused on lipoprotein metabolism in the liver and small intestine. He has published extensively in leading journals such as J Biol Chem, J Cell Sci, J Lipid Res, Biochem J. etc. His work has been continuously supported by the American Heart Association and the National Institutes of Health (NIH) R01 grants since 2007.
He has received multiple honors, including the UCF Reach for the Stars Award, Outstanding Faculty Member Award, etc. Dr. Siddiqi also serves as an Ad Hoc reviewer for multiple NIH study sections, VA Merit Review Panel, Israel Science Foundation and Medical Research Council, London, UK and numerous high-impact journals.
Research:
Dr. Siddiqi’s research investigates the cellular and molecular mechanisms governing lipoprotein metabolism in the small intestine and liver, with emphasis on very-low-density lipoprotein (VLDL) transport and secretion. His laboratory discovered the VLDL transport vesicle (VTV) – a specialized ER-derived vesicle responsible for the transport of nascent VLDL to the Golgi apparatus in hepatocytes. This discovery established a new paradigm for understanding the intracellular trafficking of lipoproteins.
More recently, his group identified a post-Golgi VLDL transport vesicle (PG-VTV) that mediates the export of mature VLDL to the plasma membrane. Ongoing NIH-funded research in his lab aims to define the molecular machinery and protein regulators responsible for VLDL packaging, trafficking, and secretion, with the long-term goal of identifying novel therapeutic targets to reduce plasma LDL levels and mitigate dyslipidemia.
Dr. Siddiqi’s lab integrates approaches in cell biology, lipid biochemistry, proteomics, and molecular genetics to explore how lipid transport dysregulation contributes to metabolic diseases, insulin resistance, and cardiovascular disorders. His group is also investigating how pharmacologic and dietary interventions can modulate hepatic lipid trafficking to improve metabolic health.
Recent Publications
1. Sekhar Vandana, Andl Thomas, Shadab A. Siddiqi*. Loss of SVIP Results in Metabolic Reprograming and Increased Retention of Very-Low-Density Lipoproteins in Hepatocytes. Int J Mol Sci. (2025) Aug 1; 26(15):7465.
2. Kayli Winterfeldt, Fahim R Tasin and Shadab A. Siddiqi*. Establishing the Role of Liver Fatty Acid-Binding Protein in Post-Golgi Very-Low-Density Lipoprotein Trafficking Using a Novel Fluorescence-Based Assay. Int J Mol Sci. (2025) Mar 7; 26(6):2399.
3. Winterfeldt K, Tasin FR, Sekhar V, Shadab A. Siddiqi*. Fenofibrate Treatment Inhibits Very-Low-Density Lipoprotein Transport Vesicle Formation by Reducing Sar1b Protein Expression. Int J Mol Sci. (2025) May; 26(10):4720.
4. Ashiqueali SA, Schneider A, Zhu X, Juszczyk E, Mansoor MAM, Zhu Y, Fang Y, Zanini BM, Garcia DN, Hayslip N, Medina D, McFadden S, Stockwell R, Yuan R, Bartke A, Zasloff M, Siddiqi Shadab, Masternak MM. Early life interventions metformin and trodusquemine metabolically reprogram the developing mouse liver through transcriptomic alterations. Aging Cell (2024) Sep; 23(9):e14227.
5. Sarah A Ashiqueali, Xiang Xu, Denise Weisenborn, Adam Gesing, Augusto Schneider, Sarah A Noureddine, Michal M Masternak, Shadab A. Siddiqi*. Calorie Restriction and Life-Extending Mutation Downregulate miR-34a to Facilitate Lipid Metabolism in the Liver); Experimental Gerontology (2024) 194:112506.
6. Ryan Clay, Shaila Siddiqi and Shadab A. Siddiqi*. Vitamin E Reduces Hepatic VLDL Secretion by Disrupting its ER-to-Golgi Transport; Can J Physiol Pharmacol. (2023) Nov 1;101(11):554-564.
7. Simeon Thibeaux, Shaila Siddiqi, Olga Zhelyabovska, Faisal Moinuddin, Michal M. Masternak and Shadab A. Siddiqi*. Cathepsin B regulates hepatic lipid metabolism through liver fatty acid binding protein cleavage. J Biol Chem. (2018); 293(6)1910-1923.
8. Siddiqi S, Zhelyabovska O, Shadab A. Siddiqi*. Reticulon 3 regulates very low density lipoprotein secretion by controlling very low-density lipoprotein transport vesicle biogenesis. Can J Physiol Pharmacol. (2018); 96(7):668-675.
9. Samata Tiwari, Shaila Siddiqi, Olga Zhelyabovska and Shadab A. Siddiqi*. Silencing of SVIP Protein Reduces VLDL Secretion from Rat Hepatocytes by Disrupting its ER-to-Golgi Trafficking. J Biol Chem. (2016); 291(24): 12514-26.
10. Hossain T, Riad A, Siddiqi S, Parthasarathy S and Shadab A. Siddiqi*. Mature VLDL Triggers the Biogenesis of a Distinct Vesicle from the Trans Golgi Network for its Export to the Plasma Membrane. Biochem J. (2014); Apr 1;459(1):47-58.
11. Samata Tiwari, Shaila Siddiqi, and Shadab A. Siddiqi*. CideB Protein is Required for the Biogenesis of Very Low-density Lipoprotein (VLDL) Transport Vesicle. J Biol Chem. (2013); 288(7): 5157-65.
12. Abdul Rahim, Erika Nafi-valencia, Shaila Siddiqi, Riyaz Basha, Carl Runyon and Shadab A. Siddiqi*. Proteomic Analysis of VLDL Transport Vesicle (VTV). J Proteomics (2012); 75:2225-2235.
13. Yellaturu CR , Deng X, Cagen LM, Mansbach 2nd CM, Siddiqi S A, Park EA, Raghow R, Elam MB. Insulin enhances posttranslational processing of nascent SREBP-1c by promoting its phosphorylation and association with COPII vesicles. J. Biol. Chem. (2009) 284(12):7518-32.
14. Shadab A. Siddiqi*. VLDL exits from the endoplasmic reticulum in a specialized vesicle, the VLDL-transport vesicle in rat primary hepatocytes. Biochem J. (2008) 413(2): 333-342.
15. Shadab A. Siddiqi and Charles. M. Mansbach II. PKCz Mediated Phosphorylation Controls the Budding of the Pre-Chylomicron Transport Vesicle. J. Cell Sci. (2008) 121:2327-38.
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