News of Note

Research Grants Awarded

 

Dr. Ken Teter was awarded a supplement to his project “THE “DISAGGREGASE” MECHANISM OF HOLOTOXIN DISASSEMBLY BY PROTEIN DISULFIDE ISOMERASE

Dr. Debopam Chakrabarti received an NIH R01 grant for his project entitled, NOVEL ANTIMALARIALS FROM FUNGI.

 

 

 

 

Dr. Alex Cole has been awarded an R21 grant for his project entitled, “BACTERIAL VAGINOSIS INDUCES MATRIX METALLOPROTEINASES THAT ENHANCE HIV INFECTION IN WOMEN.”

Publication and Research Highlights

Dr. Chakrabarti’s research group reports the discovery of fast-acting curative aryvinylquinoline antimalarial compounds in a recent article in the Journal of Medicinal Chemistry.

 

 

 

 

Dr. Debopam Chakrabarti and his collaborator’s publication is on the cover of February Issue of ACS Infectious Diseases. This cover shows a novel antiplasmodial agent attacking malaria parasites in infected red blood cells. This new compound was re-engineered from the indole alkaloid yohimbine using a ring distortion chemical synthesis reported by Huigens, Chakrabarti and co-workers”.

Dr. Sean Moore’s research group reported the mechanistic insight of translational frameshifting as a result of ribosomes colliding in the Proceedings of the National Academy of Sciences. The figure shows that stalled ribosomes have higher E-site occupancy in the absence of ribosomal protein bL9.

Dr. Saleh Naser’s research has been highlighted on the cover of December 2019 issue of Microorganisms. The study clearly associated Mycobacterium avium subspecies paratuberculosis with osteoporosis in autoimmune disease patients, including rheumatoid arthritis, with key SNPs in the TNF receptor superfamily gene.

Student Accomplishments

Michelle Cherne successfully defended her PhD. dissertation work (Dr. Alex Cole, supervisor) entitled “ HIV Infection in Women: Novel Approaches for Prevention”. Congrats to Dr. Cherne!

Division Seminar Series

October 7, 2020

A Conditional Protein Degradation System to Study Essential Gene Function in Cryptosporidium parvum

Dr. Hadi Hasan Choudhary, Postdoctoral Associate, D. Chakrabarti Lab

Recent Division Publications

Huang G, Solano CM, Melendez J, Yu-Alfonzo S, Boonhok R, Min H, Miao J, Chakrabarti D, Yuan Y. Discovery of fast-acting dual-stage antimalarial agents by profiling pyridylvinylquinoline chemical space via copper catalyzed azide-alkyne cycloadditions. Eur J Med Chem. 2020 Oct 4;209:112889. doi: 10.1016/j.ejmech.2020.112889. PMID: 33045660

Huang G, Murillo Solano C, Melendez J, Shaw J, Collins J, Banks R, Arshadi AK, Boonhok R, Min H, Miao J, Chakrabarti D, Yuan Y. Synthesis, Structure-Activity Relationship, and Antimalarial Efficacy of 6-Chloro-2-arylvinylquinolines. J Med Chem. 2020 Oct 6. doi: 10.1021/acs.jmedchem.0c00858. PMID: 32959656

AlQasrawi D, Qasem A, Naser SA. Divergent Effect of Cigarette Smoke on Innate Immunity in Inflammatory Bowel Disease: A Nicotine-Infection Interaction. Int J Mol Sci. 2020 Aug 13;21(16):E5801. doi: 10.3390/ijms21165801.PMID: 32823518 

Serrano A, Qiao X, Matos JO, Farley L, Cilenti L, Chen B, Tatulian SA, Teter K. Reversal of Alpha-Synuclein Fibrillization by Protein Disulfide Isomerase. Front Cell Dev Biol. 2020 Jul 30;8:726. doi: 10.3389/fcell.2020.00726. eCollection 2020.PMID: 32850841 

Keewan E, Naser SA. Notch-1 Signaling Modulates Macrophage Polarization and Immune Defense against Mycobacterium avium paratuberculosis Infection in Inflammatory Diseases. Microorganisms. 2020 Jul 5;8(7):1006. doi: 10.3390/microorganisms8071006.PMID: 32635645 

Azarian T, Huang IT, Hanage WP. Structure and Dynamics of Bacterial Populations: Pangenome Ecology. 2020 May 1. In: Tettelin H, Medini D, editors. The Pangenome: Diversity, Dynamics and Evolution of Genomes [Internet]. Cham (CH): Springer; 2020.PMID: 32633912 

Jayakumar JM, Shapiro OH, Almagro-Moreno S. Improved Method for Transformation of Vibrio vulnificus by Electroporation. Curr Protoc Microbiol. 2020 Sep;58(1):e106. doi: 10.1002/cpmc.106.PMID: 32614522

M Jayakumar J, Balasubramanian D, Reddi G, Almagro-Moreno S. Synergistic role of abiotic factors driving viable but non-culturable Vibrio cholerae. Environ Microbiol Rep. 2020 Aug;12(4):454-465. doi: 10.1111/1758-2229.12861.PMID: 32542975

Yakobov N, Fischer F, Mahmoudi N, Saga Y, Grube CD, Roy H, Senger B, Grob G, Tatematsu S, Yokokawa D, Mouyna I, Latgé JP, Nakajima H, Kushiro T, Becker HD. RNA-dependent sterol aspartylation in fungi. Proc Natl Acad Sci U S A. 2020 Jun 30;117(26):14948-14957. doi: 10.1073/pnas.2003266117. Epub 2020 Jun 15.PMID: 32541034

Azarian T. The Importance of Pathogen Whole-Genome Sequencing in Evaluating Interventions to Reduce the Spread of Multi-drug Resistant Organisms in the Healthcare Setting. Clin Infect Dis. 2020 Jun 6:ciaa724. doi: 10.1093/cid/ciaa724. Online ahead of print.PMID: 32505133 

AlQasrawi D, Abdelli LS, Naser SA. Mystery Solved: Why Smoke Extract Worsens Disease in Smokers with Crohn’s Disease and Not Ulcerative Colitis? Gut MAP! Microorganisms. 2020 May 2;8(5):E666. doi: 10.3390/microorganisms8050666.

Mavian C, Paisie TK, Alam MT, Browne C, Beau De Rochars VM, Nembrini S, Cash MN, Nelson EJ, Azarian T, Ali A, Morris JG Jr, Salemi M. Toxigenic Vibrio cholerae evolution and establishment of reservoirs in aquatic ecosystems. Proc Natl Acad Sci U S A. 2020 Apr 7;117(14):7897-7904. doi: 10.1073/pnas.1918763117.

Kennedy SJ, Cella E, Jubair M, Azarian T, Baker BJ, Shaw LN. Draft Genome Sequence of Verrucosispora sp. Strain CWR15, Isolated from a Gulf of Mexico Sponge. Microbiol Resour Announc. 2020 Mar 26;9(13):e00176-20. 

Meszaros VA, Miller-Dickson MD, Baffour-Awuah F Junior, Almagro-Moreno S, Ogbunugafor CB.  Direct transmission via households informs models of disease and intervention dynamics in cholera. PLoS One. 2020 Mar 12;15(3):e0229837. doi: 10.1371/journal.pone.0229837. 

Cherne MD, Cole AL, Newberry L, Schmidt-Owens M, Deichen M, Cole AM. Matrix Metalloproteinases Expressed in Response to Bacterial Vaginosis Disrupt the Endocervical Epithelium, Increasing Transmigration of HIV. Infect Immun. 2020 Mar 23;88(4). pii: e00041-20. doi: 10.1128/IAI.00041-20.

Paciaroni NG, Perry DL 2nd, Norwood VM 4th, Murillo-Solano C, Collins J, Tenneti S, Chakrabarti D*, Huigens RW 3rd*. Re-Engineering of Yohimbine’s Biological Activity through Ring Distortion: Identification and Structure-Activity Relationships of a New Class of Antiplasmodial Agents. ACS Infect Dis. 2020 Jan 16. doi: 10.1021/acsinfecdis.9b00380.

Arshadi AK, Salem M, Collin, J, Yuan JD, Chakrabarti D. DeepMalaria: Artificial Intelligence Driven Discovery of Potent Antiplasmodials. Front. Pharmacol. 15 January 2020. doi: 10.3389/fphar. 2019.01526.

Keewan E, Naser SA. The Role of Notch Signaling in Macrophages during Inflammation and Infection: Implication in Rheumatoid Arthritis? Cells. 2020 Jan 2;9(1). pii: E111. doi: 10.3390/cells9010111.

Naser A, Odeh AK, Sharp RC, Qasem A, Beg S, Naser SA. Polymorphisms in TNF Receptor Superfamily 1B (TNFRSF1B:rs3397) are Linked to Mycobacterium avium paratuberculosis Infection and Osteoporosis in Rheumatoid Arthritis. Microorganisms. 2019 Dec 4;7(12). pii: E646. doi: 10.3390/microorganisms7120646.

Cherubin P, Fidler D, Quiñones B, Teter K. Bimodal Response to Shiga Toxin 2 Subtypes Results from Relatively Weak Binding to the Target Cell. Infect Immun. 2019 Nov 18;87(12). pii: e00428-19. doi: 10.1128/IAI.00428-19.

Smith AM, Costello MS, Kettring AH, Wingo RJ, Moore SD. Ribosome collisions alter frameshifting at translational reprogramming motifs in bacterial mRNAs. Proc Natl Acad Sci U S A. 2019 Oct 22;116(43):21769-21779. doi: 10.1073/pnas.1910613116.

Miller-Dickson MD, Meszaros VA, Almagro-Moreno S, Brandon Ogbunugafor C. Hepatitis C virus modelled as an indirectly transmitted infection highlights the centrality of injection drug equipment in disease dynamics. J R Soc Interface. 2019 Sep 27;16(158):20190334. doi: 10.1098/rsif.2019.0334.

Prosperi M, Salemi M, Azarian T, Milicchio F, Johnson JA, Oliva M. Unexpected Predictors of Antibiotic Resistance in Housekeeping Genes of Staphylococcus Aureus. ACM BCB. 2019 Sep;2019:259-268. doi: 10.1145/3307339.3342138.

Guyette J, Evangelista B, Tatulian SA, Teter K. Stability and Conformational Resilience of Protein Disulfide Isomerase. Biochemistry. 2019 Aug 27;58(34):3572-3584. doi: 10.1021/acs.biochem.9b00405.