About Dr. Manish Gupta

Research Interests

Recent studies suggest that cardiovascular disease (CVD) is the number one killer disease in USA and globally. So, a better understanding is required to prevent CVD and heart failure.  Using cell culture, mouse model, macaque model and clinical samples the Gupta Laboratory’s research focus is to understand the molecular mechanism of cardiomyopathy and heart failure. A key area of research study involves identifying the mechanism of HIV and antiretroviral mediated cardiotoxicity. In addition, the Gupta Laboratory is interested in identifying novel strategies to overcome cardiotoxicity associated with antiretroviral drugs.

 

Current Lab Interests

  1. Investigating the role of post-translational modifications (Ubiquitination, SUMOylation, phosphorylation, acetylation) in heart function and cardiac disease.
  2. Structure-function analysis of sarcomeric proteins.
  3. Understanding the role of HIV-1 and antiretroviral drugs in mitochondrial quality control, cellular energy metabolism and development of cardiomyopathy.
  4. CRISPR/CAS9 mediated correction of human genetic disease.
  5. Chaperone-mediated regulation of autophagy.
  6. Role of environmental stress in epigenetic changes and metabolic adaptation.
  7. Development of cell culture and animal models to understand the mechanism of human Alzheimer disease.

 

Education, Training, and Research

  • Instructor (Research)- Center for Translational Medicine, Lewis Katz School of Medicine at Temple University, Philadelphia, PA, USA
  • Postdoctoral Fellow (Mentor: Jeffrey Robbins, Ph.D.)- Division of Molecular Cardiovascular Biology, Cincinnati Children’s Hospital Medical Center, Cincinnati, OH, USA
  • Ph.D. (Mentor: Brahm Shanker Srivastava, Ph.D.) at Jawaharlal Nehru University, New Delhi and Department of Microbiology, Central Drug Research Institute (CDRI), Lucknow, India
  • MSc. with specialization in Microbiology: The University of Burdwan, West Bengal, India

 

Memberships

American Heart Association

American Physiological Society

 

Selected recent publications

Cheung JY, Gordon J, Wang J, Song J, Zhang XQ, Prado FJ, Shanmughapriya S, Rajan S, Tomar D, Tahrir FG, Gupta MK, Knezevic T, Merabova N, Kontos CD, McClung JM, Klotman PE, Madesh M, Khalili K, Feldman AM. Mitochondrial dysfunction in human immunodeficiency virus-1 transgenic mouse cardiac myocytes. J Cell Physiol. 2019 Apr;234(4):4432-4444. doi: 10.1002/jcp.27232. Epub 2018 Sep 7. PubMed PMID: 30256393; PubMed Central PMCID: PMC6318060.

Gupta MK, Gordon J, Glauser GM, Myers VD, Feldman AM, Cheung JY, Khalili K. Lamin B is a target for selective nuclear PQC by BAG3: implication for nuclear envelopathies. Cell Death Dis. 2019 Jan 10;10(1):23. doi: 10.1038/s41419-018-1255-9. PubMed PMID: 30631036; PubMed Central PMCID: PMC6328609.

 

Gupta MK, Kaminski R, Mullen B, Gordon J, Burdo TH, Cheung JY, Feldman AM, Madesh M, Khalili K. HIV-1 Nef-induced cardiotoxicity through dysregulation of autophagy. Sci Rep. 2017 Aug 17;7(1):8572. doi: 10.1038/s41598-017-08736-x. PubMed PMID: 28819214; PubMed Central PMCID: PMC5561171.

Gupta MK, Robbins J. Making the connections: Autophagy and post-translational modifications in cardiomyocytes. Autophagy. 2016 Nov;12(11):2252-2253. doi: 10.1080/15548627.2016.1215384. Epub 2016 Aug 29. PubMed PMID: 27573291; PubMed Central PMCID: PMC5103357.

 

Gupta MK, McLendon PM, Gulick J, James J, Khalili K, Robbins J. UBC9-Mediated Sumoylation Favorably Impacts Cardiac Function in Compromised Hearts. Circ Res.2016 Jun 10;118(12):1894-905. doi: 10.1161/CIRCRESAHA.115.308268. Epub 2016 May 3. PubMed PMID: 27142163; PubMed Central PMCID: PMC4902739.

 

Knezevic T, Myers VD, Su F, Wang J, Song J, Zhang XQ, Gao E, Gao G, Muniswamy M, Gupta MK, Gordon J, Weiner KN, Rabinowitz J, Ramsey FV, Tilley DG, Khalili K, Cheung JY, Feldman AM. Adeno-associated Virus Serotype 9 – Driven Expression of BAG3 Improves Left Ventricular Function in Murine Hearts with Left Ventricular Dysfunction Secondary to a Myocardial Infarction. JACC Basic Transl Sci. 2016 Dec;1(7):647-656. doi: 10.1016/j.jacbts.2016.08.008. PubMed PMID: 28164169; PubMed Central PMCID: PMC5289821.

Gupta MK, Gulick J, James J, Osinska H, Lorenz JN, Robbins J. Functional dissection of myosin binding protein C phosphorylation. J Mol Cell Cardiol. 2013 Nov;64:39-50. doi: 10.1016/j.yjmcc.2013.08.006. Epub 2013 Aug 31. PubMed PMID: 24001940; PubMed Central PMCID: PMC3847820.

 

Razzaque MA, Gupta M K, Osinska H, Gulick J, Blaxall BC, Robbins J. An endogenously produced fragment of cardiac myosin-binding protein C is pathogenic and can lead to heart failure. Circ Res. 2013 Aug 16;113(5):553-61. doi: 10.1161/CIRCRESAHA.113.301225. Epub 2013 Jul 12. PubMed PMID: 23852539; PubMed Central PMCID: PMC3835189.

 

Recent Publications (PubMed)

https://www.ncbi.nlm.nih.gov/myncbi/1XkMb-8mhkqQd/bibliography/public/